Journal: European journal of pharmacology

Article Title: Pharmacological modulation of transglutaminase 2 in the unilateral ureteral obstruction mouse model.

doi: 10.1016/j.ejphar.2024.177037

Figure Lengend Snippet: Fig. 5. Fibrotic markers mRNA expression in the UUO model. Fold change of mRNA expression of the extracellular matrix proteins a) fibronectin; b) collagen 1α1; c) collagen 3α1; d) the profibrotic protein TGFβ; e) and myofibroblast marker αSMA in kidneys from either sham-operated mice or UUO mice with different treatments. Data are expressed as mean ± SD. Significance was assessed using one-way ANOVA with Dunnet’s post hoc and t-test comparing the vehicle versus treatment UUO groups. Significance is represented by *. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Then, slices were incubated with the primary antibody rabbit anticollagen 3α1 (1:200; 22734-1-AP, ProteinTech) and antitransglutaminase 2 (1:250; ab421, Abcam) diluted in 1% BSA overnight at 4 ◦C.

Techniques: Expressing, Marker

Journal: European journal of pharmacology

Article Title: Pharmacological modulation of transglutaminase 2 in the unilateral ureteral obstruction mouse model.

doi: 10.1016/j.ejphar.2024.177037

Figure Lengend Snippet: Fig. 7. Collagen staining in the UUO model. a) Collagen deposition was assessed using picrosirius red under polarized light with b) representative images. Collagen is visualized in gold, and the scale bar corresponds to 100 μm. c) collagen 3α1 immunostaining with d) representative images. Red fluorescence indicates collagen 3α1 staining, whereas blue corresponds to cell nuclei. Data are expressed as mean ± SD. Significance was assessed using one-way ANOVA with Dunnet’s post hoc and t-test comparing the vehicle versus treatment UUO groups. Significance is represented by *. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Then, slices were incubated with the primary antibody rabbit anticollagen 3α1 (1:200; 22734-1-AP, ProteinTech) and antitransglutaminase 2 (1:250; ab421, Abcam) diluted in 1% BSA overnight at 4 ◦C.

Techniques: Staining, Immunostaining, Fluorescence